Stage I
- Bioactive glasses with and without spherical gold nanoparticles were synthesized proving the repeatability of the samples. After 4 months of preparation, the samples show good structural stability
- The first composites of alginate-cellulose and alginate-chitosan composites were synthesized in which glass samples were introduced. The samples were characterized structurally and morphologically
- The first in vitro tests show promising results in wound healing although the composites need optimization
Published paper
Alexandra Feraru, Zsejke-Réka Tóth, Marieta Mureșan-Pop, Monica Baia, Tamás Gyulavári, Emőke Páll, Romulus VF Turcu, Klára Magyari, Lucian Baia, Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate–Gum Arabic Composites, Polymers, 15 (8) (2023) 1844, Q1 IF:5,0.
https://www.mdpi.com/2073-4360/15/8/1844
Stage II
In the second stage of the project, the following results were obtained:
- Alginate-cellulose and alginate-chitosan composites with bioactive glass were synthesized and crosslinking between alginate and cellulose, as well as between alginate and chitosan was demonstrated. Chemical bonding is present between biopolymer composites and bioactive glasses.
- Alginate-cellulose composites are porous, have good biodegradability and can absorb a remarkable amount of water. In vitro tests proved that the composites are bioactive and non-toxic.
- Composites based on alginate and chitosan are thin films, have good biodegradability, but absorb a small amount of water.
- Preliminary in vivo tests show us promising results in skin regeneration.
Published paper
Conference presentation
1. Emma Bobu, Kata Saszet, Klara Magyari, Monica Baia, TiO 2 in biopolymer – based composites for skin protection, The XIXth edition of the International Conference “Students for students”, Cluj- Napoca, 24-30 april 2023, poster presentation
2. Alexandra Feraru, Marieta Muresan-Pop, Monica Baia, Tamas Gyulavari, Emoke Pall, Klara Magyari, Lucian Baia, Biopolymeric cryogels based on alginate-gum arabic polysaccharides as biodegradable macroporous scaffolds, 33rd Annual Conference of the European Society for Biomaterials, Davos, Switzerland 4-9 september 2023, poster presentation, https://esb2023.org/
3. Klara Magyari, Sorin M. Marza, Mirela Moldovan, Zsejke-Reka Toth, Ionel Papuc, Lucian Baia, Effect of bioative glass with spherical gold nanoparticles in diabetic wound healing, 33rd Annual Conference of the European Society for Biomaterials, Davos, Switzerland 4-9 september 2023,
poster presentation, https://esb2023.org/
4. Klara Magyari, Sorin M. Marza, Mirela Moldovan, Zsejke-Reka Toth, Ionel Papuc, Lucian Baia, Ointments containing gold nanoparticles-bioactive glass composites for diabetic wound healing, 29th International Conference on Chemistry, Targu Mures 25-28 october 2023, poster presentation
5. Zsejke-Reka Toth, Alexandra Ferar, Klara Magyari, Lucian Baia, The effect of the amount of gum arabic in the application of alginate-gum arabic composites, 29th International Conference on Chemistry, Targu Mures 25-28 october 2023, poster presentation
6. Alexandra Feraru, Zsejke-Reka Toth, Marieta Muresan-Pop, Monica Baia, Tamas Gyulavari, Emoke Pall, Klara Magyari, Lucian Baia, The influence of gum arabic on physical and biological properties in the context of alginate-based biocomposites, 5th International Conference on Physic of Advanced Materials (ICPAM-15), Sharm El Sheikh, Egipt, 19-26 november 2023, poster presentation, https://icpams.com/main/
Brief presentation (final stage)
Within the project, the obtaining of bioactive materials with a beneficial role in the healing and regeneration process in rats with induced diabetes was experimentally analyzed and successfully validated. Experimental results obtained, such as
i.) the development of new biopolymer-based hydrogels with benefits in the healing and skin regeneration process in rats with induced diabetes and healthy ones,
ii.) the establishment of a clear and appropriate experimental protocol in skin pathology,
iii.) and the tracking of distribution and remanence of the composites used in the body were used to develop interventional guidelines in the skin pathology of patients with type II diabetes.
The newly developed hydrogel dressing (Figure 1) has a significant impact on wound healing, benefiting patients with wound healing problems. Together with the reduction of healing time, as well as the elimination of the possibility of bacterial infections to which the wound is exposed, they have a significant social and economic impact: faster healing means less time spent in hospitals, lower risk of other infections, shorter recovery time – leading patients to return to work faster.
The bioactive glass-alginate-cellulose hydrogel was tested on diabetic rats. The elastic thoracolumbar medical corset developed for new pets impacts veterinary medicine, being a medical device (Figure 2). It has the role of protection, support, and fixation of the spine that can be used in rats, hamsters, or dwarf rabbits in the case of spontaneous trauma, of some conditions in the thoracolumbar region, or postoperatively in some experimental surgical interventions. The purpose of using this medical device is to prevent incisional hernias after surgery and to quickly mobilize patients postoperatively.
Emma Bobu, Zsejke-Réka Tóth, Emőke Páll, Tamás Gyulavári, Lucian Baia, Klara Magyari, Monica Baia, TiO2–Alginate–Chitosan-Based Composites for Skin Tissue Engineering Applications, Gels 10 (6) (2024), 358, 10.33910.3390/gels10060358
Sorin Marian Mârza, Robert Cristian Purdoiu, Adrian Valentin Potârniche, Mariana Tătaru, Cosmin Peştean, Andras-Laszlo Nagy, Flaviu Tăbăran, Sidonia Bogdan, Ionel Papuc, Mirela Moldovan, Zsejke-Réka Tóth, Klara Magyari, Lucian Baia, Skin regeneration in diabetic rats using gold nanoparticles-bioactive glass oil-in-water cream, submitted manuscript
Sorin M.Marza, Ionel Papuc, Klara Magyari, Lucian Baia, Corset medical toraco-lombar elastic destinat noilor animale de companie, OSIM, A/00197, 19/04/2024